I'm not sure if this works with NOD32 2.x. We always recommend to download the latest version of NOD32 for DOS.
The latest NOD32 for DOS on the downloads page is version 1, dated March 14 2004. I assume izi was asking how to update the definitions for it, and I can't see any way of doing that except by copying the nod32.000 file as fosius suggested, or equivalently by installing NOD32 for DOS to the same folder as NOD32 for Windows. The latter method works fine for me. Richard